A brief introduction to SPF rabbits produced at Center for Research, Production of Vaccines and Medical Biologicals

A. Facility / Workshop

  • The facility meets Grade D cleanroom standards, utilizing an HVAC air conditioning system and HEPA filtration.
  • Monitoring of airborne particle counts, microbial levels in the air, and air exchange frequency.
  • Temperature: 18–26°C
  • Humidity: 40–90%
  • Pressure: 5–40 Pa
  • Noise level monitoring
  • Ammonia concentration monitoring
  • Automatic lighting system

B. Breeding Stock

  • 100% of breeding stock is directly imported from Japan
  • Rabbit breed: JW (Japanese White)
  • Status: SPF (Specific Pathogen Free) — free from identified pathogenic agents

C. Feeding and Husbandry Regimen

  • Feed: LRC4 feed (imported from Oriental Yeast Company, Japan), sterilized using gamma irradiation
  • Drinking water: Supplied via an automatic water system sterilized using UV (ultraviolet) radiation, drinking water samples are analyzed periodically 1–2 times per year
  • Equipment: All equipment such as cages and feeding trays are sterilized using a steam autoclave before being brought into the animal rooms
  • Equipment that cannot be autoclaved is placed overnight in a pass box before being transferred into the animal room

D. Microbiological Control

 

  • Check once a month
    • Salmonella spp

    • Pasteurella multocida

    • Bordetella bronchiseptica

    • Mycoplasma spp

    • Coccidium (Eimeria spp)

    • Ear mange

    • Body mange

  • Check every 6 months

    • Salmonella spp

    • Pasteurella multocida

    • Bordetella bronchiseptica

    • Yersinia pseudotuberculosis

    • Tularemia

    • Tuberculosis

    • Listeria monocytogenes

    • Tyzzer’s disease

    • Treponematosis

    • Dermatophytosis

    • HVJ (Sendai virus)

    • Rabbit pox virus

    • Myxoma virus

    • Mucoid enteritis

    • Hemorrhagic colitis

    • Hepatic coccidiosis

    • Intestinal coccidiosis

    • Encephalitozoon cuniculi

    • Toxoplasma gondi

    • Cysticercus pisiformis

    • Ear mange

E. SPF Rabbits Bred at Polyvac

One-week-old baby rabbit

Two-week-old baby rabbit

Five-week-old baby rabbit